Staff scientist

1 month ago


Umeå, Sweden Umeå University Full time

Project description

and tasks 

Temperatures below or above the physiological optimum for plant growth induce temperature stress, which can cause detrimental and irreversible damage to plant growth and development. While most of the studies to date have focused on big-picture elements of plant responses to climate change, our research will provide insights into the molecular and cellular responses, to improve our mechanistic understanding of plant acclimation to heat and cold stress. The project will mainly work with the root of the model organism Arabidopsis thaliana and will combine state-of-the-art approaches, e.g. various microscopy techniques, molecular and cell biology and biochemistry. The work includes high-resolution confocal microscopy and a detailed analysis plasma membrane composition and dynamics. The tasks also include the performing immunolocalizations as well as finding interactors by pulling down proteins that can regulate PN mediated auxin transport.

Qualifications

We are seeking a highly motivated plant biologist with excellent skills in confocal microscopy. A doctoral degree in molecular biology, plant physiology, biochemistry, microbiology, or equivalent is required. Prior expertise in spectroscopic techniques such as FCS and FCCS, hands-on experience with Dynamic Profiler, and proficiency in super-resolution (Airyscan) and Light sheet microscopy is also a requirement. The candidate must have demonstrated expertise in in situ whole mount immunolocalizations and proficiency in biochemical techniques like western blot and Co-IP, which are necessary for this project. The ideal candidate must be familiar with Arabidopsis thaliana root and have a good knowledge of English. Moreover, the candidate must be capable of independently planning, executing and evaluating experiments or theoretical work. Enthusiasm, responsibility, and excellent collaborative skills are important attributes for the position. Previous experience with crop roots is an asset.



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